|
KMID : 1134820120410091326
|
|
Journal of the Korean Society of Food Science and Nutrition
2012 Volume.41 No. 9 p.1326 ~ p.1330
|
|
|
Detection of Clostridium difficile by Loop-Mediated Isothermal Amplification
|
|
In Ye-Won
Ha Su-Jeong
Yang Seung-Kuk
Oh Se-Wook
|
|
|
Abstract
|
|
|
|
This study was conducted to develop a loop-mediated isothermal amplification (LAMP) method for the detection of Clostridium difficile. The tested target gene was 16S ribosomal RNA. Five different LAMP primer sets were designed, and LAMP was performed. All primer sets targeting the 16S rRNA gene (BIP, FIP, B3, F3, LF, PF) were determined as positive in tcdA-positive, tcdB-postive (A+B+) and tcdA-negative, tcdB-negative (A-B-) Clostridium difficile strains. As the LAMP reaction took less than 80 min and did not require expensive machine such as thermocycler, it can be used as a rapid and simple detection method for foodborne pathogens.
|
|
|
KEYWORD
|
|
|
Clostridium difficile, LAMP, rapid detection, 16S rRNA gene
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|